2021 Fiscal Year Final Research Report
Establishing new control methods for bacterial wilt and verticillium wilt, which are difficult-to-control soil-borne diseases of tomatoes
| Project/Area Number |
19H03091
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 41050:Environmental agriculture-related
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| Research Institution | Prefectural University of Kumamoto |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中原 浩貴 鳥取大学, 乾燥地研究センター, 特別研究員(PD) (30828364)
近藤 謙介 鳥取大学, 農学部, 准教授 (70403376)
森 太郎 滋賀大学, 教育学部, 准教授 (90725053)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 青枯病 / 半身萎凋病 / 土壌伝染性病害 / 生物的防除 / 鉄資材 / 低濃度エタノール / 病原性の低下・非病原化 |
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| Outline of Final Research Achievements |
Through this study, it was clarified that the PC (phenotypic conversion) of R. Solanacearum is induced by the addition of citric acid alone or the mixed addition of citric acid and iron compound even in three types of soil media. In the application of low concentrations of ethanol to soil contaminated with R. Solanacearum, the frequency of PC was affected by incubation temperature, ethanol concentration, and soil pH. Furthermore, the effects on soil microbes and diversity varied with soil type. In tomatoes inoculated with a PC mutant, expression of disease defense-related genes was induced in roots and leaves. The effect of soil mixing treatment of silica iron (iron compound) on the growth of tomatoes was investigated. In the spring-summer crops, there were no differences in comparisons with the control and 1.2 t/10 a applied silica iron treatment. In the summer-autumn crops, there were no differences in comparisons with control and 2.4 t/10 a applied silica iron treatment．
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| Free Research Field |
環境農学
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| Academic Significance and Societal Importance of the Research Achievements |
作物生産では化学農薬を使用しない等、環境に優しい農業技術の開発が望まれている。化学農薬に代わる農業技術として、生物農薬の開発が急務である。本研究では、安価で、安全なクエン酸や鉄化合物を利用して、土壌伝染性病害である青枯病細菌をPC化（病原性の低下、非病原化すること）することで、青枯病や半身萎凋病を防除するこができることを明らかにした。本防除法の特徴は、土壌の「殺菌・消毒」ではなく、細菌と植物の生態的な特性を組み合わせたところにある。本研究がさらに発展することで、食の安全・安心や作業性・経済性等の観点からの新たな土壌病害防除法の開発が進むと考える。
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