2021 Fiscal Year Final Research Report
Structure basis for multidrug resistance efflux pump spanning both membranes in Pseudomonas aeruginosa
| Project/Area Number |
19H03167
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Osaka University |
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Principal Investigator |
Yamashita Eiki 大阪大学, 蛋白質研究所, 准教授 (00294132)
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| Co-Investigator(Kenkyū-buntansha) |
中川 敦史 大阪大学, 蛋白質研究所, 教授 (20188890)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 膜蛋白質の物質輸送 / クライオ電子顕微鏡単粒子解析 / X線結晶構造解析 |
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| Outline of Final Research Achievements |
The efflux pump MexAB-OprM involved in multidrug resistance in Pseudomonas aeruginosa is composed of the transporter MexB in the inner membrane, the adapter protein MexA in the periplasmic region, and the outer membrane channel protein OprM in the outer membrane and is a giant membrane protein complex that spans two membranes. In this study, we succeeded for the structure analysis of the MexAB-OprM complex in the absence and presence of antimicrobials and clarified the mechanism of complex formation.
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| Free Research Field |
放射光構造生物学
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| Academic Significance and Societal Importance of the Research Achievements |
日和見細菌である緑膿菌は、健常者には感染症を発症しないが、免疫不全患者では重篤な症状をもたらす。特に多種の抗菌剤に耐性を持つ緑膿菌（多剤耐性緑膿菌）は院内感染を引き起こし社会問題の一つとなっている。多剤耐性化の原因の一つとして考えられている異物排出タンパク質複合体の構造は、新しい作用機序を持つ抗菌剤の開発へと繋がる
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