Comprehensive study on the post-Golgi trafficking pathway, mediated by diverse Rab family proteins

2023 Fiscal Year Final Research Report

• Project/Area Number: 19H03181
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43030:Functional biochemistry-related
• Research Institution: Osaka University
• Principal Investigator: Yoshimura Shin-ichiro 大阪大学, 大学院医学系研究科, 講師 (60584521)
• Co-Investigator(Kenkyū-buntansha): 岩野 智彦 山梨大学, 大学院総合研究部, 助教 (10442930)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: Rab / ゴルジ体 / トランスゴルジネットワーク / 一次繊毛

Outline of Final Research Achievements

More than half of 60 Rab proteins which are encoded by the mammalian genome, localize at the post-Golgi compartment. In this study, we identified and analyzed post-Golgi Rab-biding effector proteins.
We revealed that Rab6 and its novel binding proteins, Oxr1 and Ncoa7 localize on Golgi apparatus and trans-Golgi network (TGN) and negatively regulate V-ATPase activity. In addition, we found that Rab8 and its binding effector, EHBP1L1 localize on ciliary vesicle, which appears in early primary ciliogenesis. We also showed that EHBP1L1 cooperates with its binding proteins, CD2AP and CIN85, promote actin polymerization resulting in shortening the length of primary cilia.

Free Research Field

分子細胞生物学

Academic Significance and Societal Importance of the Research Achievements

我々は本研究の成果を世界に先駆けて論文として発表済み、あるいは発表予定であり、その点ではコミュニティーへの貢献は大きいと考えている。また我々が同定したRab結合分子の一部は疾患の原因となることが明らかになっており、本研究により当該疾患の理解が深まることが期待されることからその点でも社会的意義があると考えられる。