Super-resolution imaging with distance measurement at 1 nm accuracy in live cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H03202
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43040:Biophysics-related
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: Matsuda Atsushi 国立研究開発法人情報通信研究機構, 未来ICT研究所神戸フロンティア研究センター, 主任研究員 (20585723)
• Co-Investigator(Kenkyū-buntansha): 浜崎 淳一 国立研究開発法人情報通信研究機構, 未来ICT研究所小金井フロンティア研究センター, 主任研究員 (80399980)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 顕微鏡 / 超解像顕微鏡 / 補償光学 / 二光子顕微鏡

Outline of Final Research Achievements

Advances in super-resolution and cryo-electron microscopy revealed the detailed structures of minute organelles and highly functional protein complexes in living organisms. However, a great deal of technical development is still needed to achieve high resolution in living cells. In the course of this research, it became clear that in order to achieve these techniques, it is necessary to correct optical aberrations, which are a fundamental optical problem. In this research, we developed an innovative method that can correct optical aberrations after image acquisition.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

本研究の計算補償光学は光学的な補償光学と比較してはるかに簡便であり、実用化できれば、様々な高精度の計測が可能になると考えられる。生体深部観察の分解能を飛躍的に向上できるほか、超解像顕微鏡の発展にも貢献すると考えられる。