Dissection of cell fate regulation by non-invasive genome and epigenome analysis

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H03217
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43060:System genome science-related
• Research Institution: Kyoto University
• Principal Investigator: Watanabe Akira 京都大学, 医学研究科, 特定准教授 (60506765)
• Co-Investigator(Kenkyū-buntansha): 坂本 智子 京都大学, 医学研究科, 研究員 (70648427) ; 沖田 圭介 京都大学, iPS細胞研究所, 講師 (90512434)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: DNAコピー数 / 非侵襲的解析

Outline of Final Research Achievements

Genomic aberrations and alterations of epigenome are associated with cell differentiation and tumorigenesis. Current analysis for genome and epigenome after lysis of the cells is far from continuous observation of the status. We have established non-invasive assay by copy number analysis of cell-free DNA. The result was approximately close to DNA copy number analysis of genomic DNA obtained by lysis of the cells. We developed novel in silico tools to detect copy number mosaicism from results of the non-invasive DNA copy number analysis.

Free Research Field

ゲノム科学

Academic Significance and Societal Importance of the Research Achievements

本方法は、細胞を破砕せずにDNA状態を解析でき、一部の細胞で生じる変化を捉えることができる。すなわち、細胞培養中に生じるDNAコピー数変化を捉えられることから、再生医療用細胞の品質評価への応用が可能である。また、研究に用いる初代培養細胞や細胞株の拡大培養における品質評価も可能となっており、学術的および産業的に幅広い応用が期待できる。