2021 Fiscal Year Final Research Report
Molecular mechanism of cellular reprogramming by ribosome
Project/Area Number |
19H03235
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 44020:Developmental biology-related
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Research Institution | Kyushu University |
Principal Investigator |
Ohta Kunimasa 九州大学, 基幹教育院, 教授 (90244128)
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Co-Investigator(Kenkyū-buntansha) |
嶋村 健児 熊本大学, 発生医学研究所, 教授 (70301140)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | リボソーム / 形質転換 / 多能性 |
Outline of Final Research Achievements |
Previously, we reported that the incorporation of ribosome into mouse fibroblast cells induced the multipotency in the host cells (Ito et al., 2018). In this study, we tried to examine the molecular function of incorporated ribosome in the host cells. When ribosome was incorporated into human cancer cells, cells made clusters and stopped the cell proliferation. Therefore, ribosome transdifferentiated the cancer cells. Next, we found that the exogenous ribosome was localized in the nuclear and induced the epigenetic modification. Further, ATAC-seq analysis indicated the 22,921 acquired and the 30,146 loss chromatin areas in the ribosome incorporated cells. Further, by the single cell analysis, the ribosome incorporated cells are categorized into 4 groups by time series.
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Free Research Field |
幹細胞生物学
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Academic Significance and Societal Importance of the Research Achievements |
タンパク質合成装置として知られているリボソームを細胞に投与すると、リボソームは細胞質内だけでなく、核内にも取り込まれていた。宿主細胞の核内では、エピジェネティクな修飾が誘導され、様々な遺伝子の発現パターンが変化していた。さらに、リボソームを取り込んだ細胞は、時系列的に、4つの異なった細胞種へと変化することが示された。本研究では、リボソームの新たな役割を明らかにすることが出来た。
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