Development of whole-organ 3D staining method for the analysis of protein expression, modification, and activity

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03413
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: Juntendo University (2021); The University of Tokyo (2019-2020)
• Principal Investigator: Etsuo Susaki 順天堂大学, 大学院医学研究科, 教授 (10444803)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 3次元組織学 / 組織透明化 / 3次元イメージング

Outline of Final Research Achievements

The purpose of this research was to establish an original organ-scale multicolor 3D staining and observation technique. During the research, we invented a new, high-performance tissue 3D staining protocol named CUBIC-HistoVIsion (CUBIC-HV) based on our finding that the biological tissue can be defined as a kind of gel. The published paper gathered worldwide attention and was selected as one of the 2020 Top 50 Life and Biological Sciences Articles in the journal. We also conducted many collaboration research by using the newly developed technology.

Free Research Field

生化学、組織化学、バイオイメージング

Academic Significance and Societal Importance of the Research Achievements

本研究課題は、世界最高性能の3次元組織学技術を確立するとともに、組織内のタンパク質の状態を網羅的に解析する新しい研究アプローチの確立に貢献した。この方法は、創薬や臨床病理学を始めとする、基礎研究以外の様々な分野での活用が期待され、実際に応用も進められている。