Suppression of hepatocarcinogenesis based on the real-time blood-borne visualization of viral genome integration and biofunctional information

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H03568
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 52010:General internal medicine-related
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Fumio Itoh 聖マリアンナ医科大学, 医学部, 教授 (90223180)
• Co-Investigator(Kenkyū-buntansha): 山本 博幸 聖マリアンナ医科大学, 医学研究科, 教授 (40332910) ; 渡邊 嘉行 聖マリアンナ医科大学, 医学部, 講師 (90329243) ; 渡邊 綱正 聖マリアンナ医科大学, 医学部, 准教授 (20338528)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ウイルスゲノム組み込み / HBV / 生体機能情報 / 血液診断 / 肝癌

Outline of Final Research Achievements

Integration of hepatitis B virus (HBV) DNA into the human genome plays an important role in HBV-related hepatocellular carcinoma. We have successfully optimized the analysis of cancer tissue specific integrated HBV DNA into human genome using formalin fixed paraffin embedded (FFPE) samples. For a diagnostic use of blood-borne HBV DNA-integrated human genome, we have characterized the molecular details of shedding of HBV DNA-integrated human genomes in liver cancer cell lines. We have also successfully developed a biosensing method of HBV DNA-integrated human genome and related biofunctional information. These results can be applied to suppression of hepatocarcinogenesis based on the real-time blood-borne visualization of viral genome integration and biofunctional information.

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

今後、採血のみでHBV DNA組み込み部位および関連する生体機能情報の可視化が可能になることにより、癌超早期診断や発癌リスク判定などの個別化医療を可能にしうることから学術的意義が大きい。さらに、肝癌サバイバーやウイルス持続感染者（国内150万人）のQOL改善を通して国民の健康福祉の増進に寄与しうることから社会的意義も大きい。