Biliary homeostasis regulated by extracellular vesicles and its dysregulation resulting in liver diseases

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03632
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Yamagata University
• Principal Investigator: Ueno Yoshiyuki 山形大学, 医学部, 教授 (70282126)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 胆汁 / 細胞外小胞 / 胆汁うっ滞 / 胆管癌 / 原発性硬化性胆管炎

Outline of Final Research Achievements

(1) To establish a method for separating EV from bile sample, we conducted joint research with Miraka Research Institute, developed a therapeutic drug, and devised the optimum method for separation from bile. This method revealed that bile samples of human bile stasis liver disease have different compositions and biomarkers for each disease. (2) Separated EV is added from the lumen side of cultured bile duct epithelial cells, and changes in the cells are examined. Clarified. Furthermore, (3) it was found that the basal EV has a fibrosis-enhancing effect that has a more fibrotic-enhancing effect than the normal state in a human disease (human PSC in vitro model stimulated with interferon and bile acid).

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

本研究により、より効率が高く純度の高い胆汁中EVの分離法が開発された。本法を用いることにより、新たな疾患バイオマーカーや、創薬につながるデリバリー法の開発が可能となることが期待される。