2021 Fiscal Year Final Research Report

Elucidation and control of pancreatic cancer-stroma interaction by artificial cancer tissue construction

Research Project

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Project/Area Number	19H03718
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 55010:General surgery and pediatric surgery-related
Research Institution	National Cancer Center Japan (2020-2021) The University of Tokyo (2019)
Principal Investigator	Sekine Keisuke 国立研究開発法人国立がん研究センター, 研究所, ユニット長 (00323569)
Co-Investigator(Kenkyū-buntansha)	谷口英樹東京大学, 医科学研究所, 教授 (70292555)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	オルガノイド / 癌
Outline of Final Research Achievements	In this study, we performed in vivo spatial omics analysis for the purpose of clarifying pancreatic cancer cell-stromal interactions. Furthermore, primary cells were established from clinical specimens, and a single-cell level comprehensive gene expression analysis was performed on the established primary cells. By conducting informatics analysis that integrates these data, we carried out ligand-receptor analysis and intracellular signal network analysis related to transcription factor networks and cell-cell interactions. We succeeded in identifying a possible transcription factor network and candidate molecules for multiple pancreatic cancer cell-interstitial interactions.
Free Research Field	腫瘍細胞生物学、幹細胞生物学
Academic Significance and Societal Importance of the Research Achievements	膵癌は極めて予後の悪い難治癌であり、本邦では肝癌を抜いて第4位となっており、2030年までには第２位となるとの予測もあるなど、予後の改善に向けた開発が社会的要求が高いといえる。本研究では膵癌の進展に必須と考えられる膵癌細胞と間質の間におこる相互作用に関与すると考えられる転写因子ネットワークの同定と複数の膵癌細胞-間質相互作用の候補分子の同定に成功した。今後これらの分子を標的とした機能解析と創薬開発により、膵癌の予後改善に貢献する可能性が期待される。