2021 Fiscal Year Final Research Report

Elucidation of the molecular mechanisms underlying mesenchymal transition of glioma cells using methylation array analysis

Research Project

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Project/Area Number	19H03773
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 56010:Neurosurgery-related
Research Institution	University of Miyazaki
Principal Investigator	Takeshima Hideo 宮崎大学, 医学部, 教授 (70244134)
Co-Investigator(Kenkyū-buntansha)	横上聖貴宮崎大学, 医学部, 准教授 (40284856) 山下真治宮崎大学, 医学部, 助教 (40468046) 渡邉孝宮崎大学, 医学部, 講師 (90573337)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	膠肉腫 / 間葉型形質転換 / DNAメチル化アレイ解析 / Classifier / CRC culture
Outline of Final Research Achievements	To elucidate the molecular mechanism underlying mesenchymal transition of glioma cells, we focused on the epigenetic change in glioma cells. We investigated gliosarcoma, since this tumor consists of epithelial components and mesenchymal components which share the same genetic alteration in the mosaic fashion. In this project, we analyzed using methylation EPIC BeadChip and Heidelberg CNS Tumor Classifier to compare both epigenetic pattern. However, we could not find any significant difference in epigenetic changes between both two components, suggesting that there might be alternative mechanism in mesenchymal transition of glioma cells.
Free Research Field	脳神経外科
Academic Significance and Societal Importance of the Research Achievements	神経膠腫の治療抵抗性の機序の１つとしてグリオーマ幹細胞の上皮間葉転換が知られているが、今回の研究のネガティブデータによりＤＮＡのメチル化を介したエピジェネティックなメカニズムは関与が薄い可能性が示唆された。これをもとに，今後の研究の方向性が、腫瘍における微小環境における細胞間の相互作用やマイクロＲＮＡの発現解析など、その他の調節系に焦点を絞るべき方向性が示唆されたものと考えられた。