Analysis of the mechanism of skin regeneration in mouse fetuses using conditional knockout mice

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03815
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Keio University
• Principal Investigator: Kishi Kazuo 慶應義塾大学, 医学部(信濃町), 教授 (40224919)
• Co-Investigator(Kenkyū-buntansha): 久保田 義顕 慶應義塾大学, 医学部(信濃町), 教授 (50348687) ; 岡部 圭介 慶應義塾大学, 医学部(信濃町), 講師 (50445350) ; 荒牧 典子 慶應義塾大学, 医学部(信濃町), 講師 (80365311)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 皮膚 / 再生 / 胎仔

Outline of Final Research Achievements

Mammalian fetuses regenerate skin wounds up to a certain age quickly and completely without scarring. We found that skin wounds on mouse fetuses up to embryonic day 13 (E13) regenerate completely, but fetal skin after E14 does not regenerate and leaves scars. By comparing the skin before and after complete skin regeneration, we identified the gene expression characteristic of each period. By regulating actin polymerization at the expression sites of keratin 14 (CK14) and keratin 17 (CK17), we verified whether or not it is possible to regenerate mouse fetuses, which normally leave scars, without leaving any scars.

Free Research Field

形成外科

Academic Significance and Societal Importance of the Research Achievements

今回の研究で、K14,K17の発現部位で、アクチンの重合を変化させても皮膚の完全再生に至ることはなかったが、表皮の遊走は完全にブロックし、これによりactin cableの形成は可能であった。皮膚の完全再生には真皮の役割も関わっているので、今後真皮の動態も同時に変化させることで、皮膚の完全再生に迫ることができるものと考えられる。