Organoid optimization for rapid bone mineralization and controlled products quality

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03837
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57040:Regenerative dentistry and dental engineering-related
• Research Institution: Okayama University
• Principal Investigator: Matsumoto Takuya 岡山大学, 医歯薬学域, 教授 (40324793)
• Co-Investigator(Kenkyū-buntansha): 山本 雅哉 東北大学, 工学研究科, 教授 (10332735)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 組織制御 / 時間空間制御 / オルガノイド / 骨組織

Outline of Final Research Achievements

While organogenesis research has been paid tremendous attention in these years, there are still many problems to be solved when applying bone organoid technology. One of the problems is "Shortening of time" and the other is "control of physical properties of products".
Here, in this study, (a) the actual bone tissue development process is reexamined from a material point of view, (b) the physical and chemical artificial environment is reproduced and constructed in vitro based on the obtained results, and (c). We tried and evaluated the control of bone organoid production using these constructed environments. As a result, we have developed an in vitro rapid mineralization methods and elucidated a part of the mechanism.

Free Research Field

歯科再生医学

Academic Significance and Societal Importance of the Research Achievements

本研究では、実際の生体骨生成過程を模倣し、再現するという新しいアプローチで骨ブロックともいえる微小骨の迅速石灰化ならびに物性制御の可能性を示すことに成功した。この骨ブロックは有機質を基材とした中で、細胞由来の石灰化物を自然析出させるものや、石灰化物を有機基材と混在してパターニングするなど、これまでにない材料といえる。この微小骨を組織再生に応用する形での医療の実現は患者のQOL回復につながり、早期の社会復帰など社会的にも大きな意義があると思われる。