Development of Bone Organoid Models Towards the Precision Prosthodontics

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03840
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Tohoku University
• Principal Investigator: Egusa Hiroshi 東北大学, 歯学研究科, 教授 (30379078)
• Co-Investigator(Kenkyū-buntansha): 新部 邦透 東北大学, 大学病院, 講師 (50468500) ; 山田 将博 東北大学, 歯学研究科, 准教授 (90549982)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: iPS細胞 / オルガノイド / 骨 / 軟骨 / 歯胚

Outline of Final Research Achievements

Recently, organoid-on-a-chip technologies, which enable us to reproduce biological function of cells and tissues at the organ level in vitro, commands considerable attention. The aim of this study was to establish culture methods for the bone/cartilage/tooth organoids-on-a-chip using induced pluripotent stem (iPS) cell technology towards creating precision prosthodontics. We established induction method of iPS cells to bone organoid using micro-space culture wells. We also found a method to induce the bone/cartilage hybrid by applying a shaking culture. Furthermore, we established a step-wise culture method to induce iPS cells to enamelblasts. These results would contribute to the production of bone/cartilage/tooth organoids-on-a-chip in the future, and may lead to the basic technology of precision prosthetics that reproduces patient hard tissues in vitro.

Free Research Field

再生医学、歯科補綴学

Academic Significance and Societal Importance of the Research Achievements

近年、個人差を適切な治療法の選択に利用する精密医療の有用性が認識されており、個人差の術前診断に向けたヒトの病態・体質を試験管内で再現・評価するモデル（オルガノイド）の開発が重要となっている。本研究は、万能細胞として知られるiPS細胞を利用して、試験管内で骨、軟骨、歯のオルガノイドの作製技術を検討したものであり、その成果は顎の骨や歯の個人差を分子生物学的に検出する基盤技術に繋がることが期待される。