Development of wound care for regulating inflammatory responses using nano-type lactic acid bacteria

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03918
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 58050:Fundamental of nursing-related
• Research Institution: Tohoku University
• Principal Investigator: Kanno Emi 東北大学, 医学系研究科, 准教授 (10431595)
• Co-Investigator(Kenkyū-buntansha): 川上 和義 東北大学, 医学系研究科, 教授 (10253973) ; 丹野 寛大 東北大学, 医学系研究科, 助教 (10755664) ; 高木 尚之 東北大学, 医学系研究科, 非常勤講師 (30569471) ; 館 正弘 東北大学, 医学系研究科, 名誉教授 (50312004) ; 丸山 良子 東北大学, 医学系研究科, 名誉教授 (10275498)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 難治性皮膚潰瘍 / 創傷治癒 / ナノ型乳酸菌 / 炎症

Outline of Final Research Achievements

The purpose of our study is to develop an “innovative skin wound care using nano-type lactic acid bacteria (heat-killed KH2).”
We analyzed percent wound closure and granulation area. We found that heat-killed KH2 contributed to the acceleration of the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1 production. In addition,
heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.

Free Research Field

基礎看護学

Academic Significance and Societal Importance of the Research Achievements

現在、重症化した皮膚潰瘍に対する治療は、医療機関に入院し、陰圧閉鎖療法等で創部感染を抑え、肉芽組織を高めた後に手術を実施するのが一般的となっている。
今回の解析により、ナノ型乳酸菌を用いた治療法が確立されれば、在宅や外来通院により、事前に良質な肉芽組織を高める治療を行い、短期間の入院での手術が可能となる。さらに創底をしっかりつくることにより、再発予防に寄与する可能性も高い。