2021 Fiscal Year Final Research Report
Degradation kinetics of various source materials of environmental DNA in sediment
| Project/Area Number |
19H04290
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 64010:Environmental load and risk assessment-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
飛野 智宏 東京大学, 大学院工学系研究科(工学部), 講師 (90624916)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 環境DNA / 底生生物 / 底泥 |
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| Outline of Final Research Achievements |
This study focused on the DNA degradation kinetics in sediment of water environment. An estuarine amphipod Grandidierella japonica was used as a test organism and the potential source materials of environmental DNA was produced to conduct a laboratory test of its biodegradation. We successfully prepared the dead bodies of juvenile amphipods and the feces of fish predating the amphipods and subjected them to the degradation test. As a result, the degradation rate of DNA in the dead bodies was significantly higher than in the fish feces and also in the products of growing phase. The result suggested the importance of predator's feces in the environmental DNA analysis of sediments.
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| Free Research Field |
都市環境工学
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| Academic Significance and Societal Importance of the Research Achievements |
環境DNA分析は環境中の何を測っているのか、を知ることは、対象生物の定量的推定や効率的なモニタリング実施において重要な情報であるが、知見が限られている。本研究では、捕食者糞中DNAはほぼ分解されず、死骸由来DNAは環境中でより分解されやすいことが示唆された。今後の調査においては捕食生物も含めた網羅的なDNA分析をすることが望ましい。
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