Development of three-dimensional complex tissue engineering based on vascular interaction

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H04452
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 90110:Biomedical engineering-related
• Research Institution: Keio University
• Principal Investigator: Sudo Ryo 慶應義塾大学, 理工学部(矢上), 教授 (20407141)
• Co-Investigator(Kenkyū-buntansha): 山下 忠紘 慶應義塾大学, 理工学部(矢上), 講師 (00827339) ; 谷下 一夫 慶應義塾大学, 理工学部(矢上), 名誉教授 (10101776) ; 三高 俊広 札幌医科大学, 医学部, 教授 (50231618)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 血管相互作用 / 三次元肝組織 / 胆管がんオルガノイド

Outline of Final Research Achievements

The regulation of vascular interaction, i.e. the interaction between blood vessels and surrounding tissues such as normal and tumor tissues, is crucial for the development of novel tissue engineering to construct a higher-order three-dimensional (3D) tissue combining multiple tissues. Therefore, in this study, we first constructed a functional vascular structure in vitro and investigated the vascular interaction based on it. We then established a 3D culture method that combines blood vessels and normal tissues (e.g. 3D liver tissue). In addition, we developed a culture model for proximal culture of vascular/lymphatic vessels and tumor tissue (e.g. cholangiocarcinoma organoids) and investigated the effects of vascular/lymphatic vessels on the growth of cholangiocarcinoma organoids.

Free Research Field

組織工学

Academic Significance and Societal Importance of the Research Achievements

本研究は、血管相互作用を基軸として、血管と三次元肝組織（実質組織）、および血管と胆管がんオルガノイド（腫瘍組織）の関係を明らかにすることによって、複数の組織が複合化した高次の三次元組織を構築する組織工学（三次元コンプレックス組織工学）の学術基盤を創生した。三次元組織工学において血管組織と実質組織、および血管組織と腫瘍組織の相互作用（血管相互作用）を解明することは極めて重要な課題であり、立体臓器の再生手法や新たながん治療戦略の立案につながる。