2022 Fiscal Year Final Research Report
Study on a regeneration mechanism of corneal stroma in a decellularized scaffold and on the methods to construct corneal stromal like architecture
| Project/Area Number |
19H04477
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | Tokyo Medical and Dental University (2021-2022)
National Institute for Materials Science (2019-2020) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
橋本 良秀 東京医科歯科大学, 生体材料工学研究所, 助教 (40638384)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 脱細胞角膜足場 / 再生メカニズム / 上皮化 / 細胞浸潤 |
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| Outline of Final Research Achievements |
Epithelial cells were seeded on the decellularized tissue in a perfusion culture system, and the culture was continued. We investigated the epithelial regeneration mechanism by scratching the epithelial layer and breaking the continuity. It was observed that epithelial cells migrated from the tip of the epithelium to the defect site and re-epithelialization progressed. While re-epithelialization progressed, there were some cases where progress of epithelialization was slow and cell infiltration into the parenchyma occurred. As a result of examining the expression of MMP and keratin by immunostaining, the expression of keratin was weak in the multi-layered epithelial layer on the decellularized tissue, and the expression of MMP continued. Cells that infiltrated the parenchyma showed marked expression of MMP, but no expression of keratin. It suggested that the corneal epithelium infiltrated into the parenchyma, and epigenetically changed the state of cell differentiation.
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| Free Research Field |
角膜再生
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| Academic Significance and Societal Importance of the Research Achievements |
角膜治療の第一選択肢は献眼によるアログラフトを使用するものであるが、献眼数が少なく輸入眼に頼るのが現状である。この状況を改善するために、豚角膜から細胞成分を除去して免疫拒絶反応を低減させて人角膜治療に用いる試みがなされている。この治療は、有望であるが、未だ詳細な再生メカニズムが分かっているわけではなく、これを解き明かすことがこの治療法の信頼性の向上につながる。脱細胞組織の上皮化、および、組織内への細胞浸潤のメカニズムなどの一部が本研究より明らかになり、今後の実用化に向けた医療デバイスとしての製品デザインに資する結果が得られたことは、学術的にも、実用上に於いても大きな意義を持つものと考えられた。
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