Development of on-chip liquid biopsy for achieving express diagnosis of cancer primary tumor

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H04489
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 90130:Medical systems-related
• Research Institution: Kumamoto University
• Principal Investigator: Nakashima Yuta 熊本大学, 大学院先端科学研究部(工), 准教授 (70574341)
• Co-Investigator(Kenkyū-buntansha): 藤原 章雄 熊本大学, 大学院生命科学研究部(医), 准教授 (70452886)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: リキッドバイオプシー / BioMEMS / がん検診

Outline of Final Research Achievements

This research objective is to develop the technique for enables diagnosis the primary cancer sites by liquid biopsy. The cells are used to the sensor for detecting the cancer. The developed device consists of a microchannel for culturing the sensing-cell and a detection area for cancer detection. We clarify that the sensing-cell produced different substances depending on the cancer type when the culture supernatant of cancer cells was injected into the culture microchannel as sample. Also, we demonstrated that the cell produced substances were able to be detected in real time by the detection area. It was also successfully detected the sensing-cell produced substances in an experiment using serum from mice implanted with cancer. These results suggested that further advancement of the developed technique could detect the primary cancer sites.

Free Research Field

バイオマイクロデバイス

Academic Significance and Societal Importance of the Research Achievements

がんを早期に発見する点において、リキッドバイオプシーは低侵襲かつ簡便、高速に検査できる点で有用であることが周知の事実であるが、既存の技術では、原発巣を特定できないことが大きな課題である。本研究の成果は、リキッドバイオプシーによってがんの原発巣を特定できる可能性を示唆した点で、がん医療における早期発見、早期治療の観点で非常に大きな社会的意義がある。また、本成果は、自身の細胞を用いた評価が可能なため、がんの診断のみならず、他の疾患への適用や、細胞応答を基にした治療薬のスクリーニングや治療方針決定に適用することができるなど、次世代医療への展開・貢献も期待でき、学術的・社会的意義を持つ。