2023 Fiscal Year Final Research Report

Establishment of an integrated locomotive science including dynamics of bone-articular cells and regulation by immune system

Research Project

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Project/Area Number	19H05654
Research Category	Grant-in-Aid for Scientific Research (S)
Allocation Type	Single-year Grants
Review Section	Broad Section I
Research Institution	The University of Tokyo
Principal Investigator	Tanaka Sakae 東京大学, 医学部附属病院, 教授 (50282661)
Co-Investigator(Kenkyū-buntansha)	橋爪洋和歌山県立医科大学, 保健看護学部, 教授 (10326382) 小松紀子東京大学, 大学院医学系研究科(医学部), 助教 (20553358) 齋藤琢東京大学, 医学部附属病院, 准教授 (30456107) 小俣康徳東京大学, 医学部附属病院, 特任准教授 (40570734) 吉村典子東京大学, 医学部附属病院, 特任教授 (60240355) 藤尾圭志東京大学, 医学部附属病院, 教授 (70401114) 岡田随象東京大学, 大学院医学系研究科(医学部), 教授 (70727411)
Project Period (FY)	2019-06-26 – 2024-03-31
Keywords	運動器科学 / 変形性関節症 / 関節リウマチ / 骨粗鬆症 / シングルセル解析
Outline of Final Research Achievements	We have established the method for single-cell analysis, isolated cells, created libraries by single-cell extraction, and performed sequencing analyses. scRNAseq was performed on synovial cells from humans and mouse models of RA and OA. Clustering of these cells based on their expression profiles identified several major groups with features of fibroblasts, macrophages, and endothelial cells and minor subsets with features of T cells, B cells, and ILCs. Single-cell analyses were also performed on synovial and tendon ligament tissues from mouse models of bone and joint diseases, such as tail suspension and Achilles tendon injury models. Combining the single-cell analyses with genome data, we revealed several subsets of genes responsible for the homeostasis of bone and joints; several candidate molecules were extracted as genes characteristic of the pathogenesis of bone and joint diseases.
Free Research Field	分子細胞生物学
Academic Significance and Societal Importance of the Research Achievements	本研究で得られた成果は、運動器の恒常性や変化を現在の最新のテクノロジーを駆使して得られた知見であり、変形性関節症や関節リウマチ、骨粗鬆症など運動器疾患の理解を深める内容となった。本研究の成果は学術的にも国内外で注目されている研究内容や成果であり、また社会に還元し得る成果であると考えられる。