2020 Fiscal Year Annual Research Report
インタラクトーム解析に向けた高速多色振動分光イメージング
| Project/Area Number |
19J22546
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| Research Institution | The University of Tokyo |
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Principal Investigator |
寿 景文 東京大学, 工学系研究科, 特別研究員(DC1)
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| Project Period (FY) |
2019-04-25 – 2022-03-31
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| Keywords | super-multiplex / stimulated Raman / fluorescence / high-speed |
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| Outline of Annual Research Achievements |
We have worked on the theme of ‘super-multiplex biological imaging’ towards different directions of development.
1. We report integrated stimulated Raman and fluorescence microscopy with simultaneous multimodal color tunability at high speed, enabling super-multiplex imaging covering diverse molecular contrasts with temporal resolution of seconds.
2. We demonstrate photoswitchable stimulated Raman scattering spectroscopy and microscopy where narrowband vibrational signatures are switched with full reversibility at high speed.
3. We developed four types of activatable Raman probes, which are targeted to different enzymes and tuned to different vibrational frequencies by isotope editing of the nitrile group.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
To achieve the initial topic of 'High-speed super-multiplex organelle imaging for interactome analysis', we have developed the integrated stimulated Raman and fluorescence microscopy with fast programmable tunability.
By exploiting the advanced imaging system, we are able to observe up to eight kinds of subceullar organelles simultaneously with temporal resolution of seconds.
Besides, we also built a novel techniques called photoswitchable stimulated Raman scattering which is desired for super-multiplex super-resolution imaging. Also, we developed new kinds of Raman probes for enzyme imaging.
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| Strategy for Future Research Activity |
In the last year, we are going to work on the publication of the achievements. Despite the journal papers we have submitted, we plan to attend more conference to share our results and build helpful connnection with other researches in different research fields.
Furthermore, we will develop novel light sources with faster and wider tunability to enhance the high-speed super-multiplex imaging more. Also, we will modify the photoswitch stimulated Raman scattering technologies towards super-multiplex super-resolution imaging which is highly promising to bring new bioligical insights.
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