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2020 Fiscal Year Annual Research Report

インタラクトーム解析に向けた高速多色振動分光イメージング

Research Project

Project/Area Number 19J22546
Research InstitutionThe University of Tokyo

Principal Investigator

寿 景文  東京大学, 工学系研究科, 特別研究員(DC1)

Project Period (FY) 2019-04-25 – 2022-03-31
Keywordssuper-multiplex / stimulated Raman / fluorescence / high-speed
Outline of Annual Research Achievements

We have worked on the theme of ‘super-multiplex biological imaging’ towards different directions of development.
1. We report integrated stimulated Raman and fluorescence microscopy with simultaneous multimodal color tunability at high speed, enabling super-multiplex imaging covering diverse molecular contrasts with temporal resolution of seconds.
2. We demonstrate photoswitchable stimulated Raman scattering spectroscopy and microscopy where narrowband vibrational signatures are switched with full reversibility at high speed.
3. We developed four types of activatable Raman probes, which are targeted to different enzymes and tuned to different vibrational frequencies by isotope editing of the nitrile group.

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

To achieve the initial topic of 'High-speed super-multiplex organelle imaging for interactome analysis', we have developed the integrated stimulated Raman and fluorescence microscopy with fast programmable tunability.
By exploiting the advanced imaging system, we are able to observe up to eight kinds of subceullar organelles simultaneously with temporal resolution of seconds.
Besides, we also built a novel techniques called photoswitchable stimulated Raman scattering which is desired for super-multiplex super-resolution imaging. Also, we developed new kinds of Raman probes for enzyme imaging.

Strategy for Future Research Activity

In the last year, we are going to work on the publication of the achievements. Despite the journal papers we have submitted, we plan to attend more conference to share our results and build helpful connnection with other researches in different research fields.
Furthermore, we will develop novel light sources with faster and wider tunability to enhance the high-speed super-multiplex imaging more. Also, we will modify the photoswitch stimulated Raman scattering technologies towards super-multiplex super-resolution imaging which is highly promising to bring new bioligical insights.

  • Research Products

    (5 results)

All 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Columbia University(米国)

    • Country Name
      U.S.A.
    • Counterpart Institution
      Columbia University
  • [Journal Article] Photoswitchable stimulated Raman scattering spectroscopy and microscopy2021

    • Author(s)
      Shou Jingwen、Ozeki Yasuyuki
    • Journal Title

      Optics Letters

      Volume: - Pages: -

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities2020

    • Author(s)
      Fujioka Hiroyoshi、Shou Jingwen、Kojima Ryosuke、Urano Yasuteru、Ozeki Yasuyuki、Kamiya Mako
    • Journal Title

      Journal of the American Chemical Society

      Volume: 142 Pages: 20701~20707

    • DOI

      10.1021/jacs.0c09200

    • Peer Reviewed / Int'l Joint Research
  • [Presentation] High-speed super-multiplex imaging of brain tissue2021

    • Author(s)
      Robert Oda
    • Organizer
      Photonics West 2021
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] Activatable型ラ マ ン プ ロ ー ブ2019

    • Inventor(s)
      浦野泰照 等5人
    • Industrial Property Rights Holder
      浦野泰照 等5人
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      20B19Z001-1

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Published: 2021-12-27  

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