2021 Fiscal Year Annual Research Report
Supramolecular interactions between proteins and bioadhesive nanoparticles
| Project/Area Number |
19J23043
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Morishita Kiyoshi 東京大学, 工学系研究科, 特別研究員(DC1)
|
|---|
| Project Period (FY) |
2019-04-25 – 2022-03-31
|
| Keywords | Supramolecular chemistry / Colloidal Assembly / DNA nanotechnology / Nanoparticles / Proteins / GroEL / Materials Science |
|---|
| Outline of Annual Research Achievements |
This year I worked on i) porous clusters formed by GroEL and gold nanoparticles (AuNPs), and ii) rugby ball self-assembly of DNA-functionalized GroEL.
In project i) I confirmed the ATPase activity of the clusters and analyzed the porous nature of the clusters by TEM, cryo TEM, and cryo-EM tomography. This quantitative approach confirmed that the clusters had discrete numbers of GroEL connecting AuNPs, as designed. The porous nature was also probed by guest loading studies, using various polymer-protein hybrids as guest species. This indicated that the cluster can accommodate guests on the order of several tens of nanometers in size, with a guest hydrodynamic diameter limit of around 45 nm. Experiments on the effect of guest polymer conformation on loading are ongoing.
In project ii) I studied the roles of various nucleotides and analogues, DNA sequence, and salt conditions in the rugby ball formation. This gave a complete understanding of the important factors for growth and supported a proposed mechanism for the assembly. I identified the alignment of GroEL units in the structure and its anisotropic nature. Current experiments are targeted to identify whether the structure is hollow.
|
| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
|
