2021 Fiscal Year Final Research Report
Novel preparation of enzyme-entrapped silica nanocapsule using sugar nanoparticle as sacrifice matrix
| Project/Area Number |
19K05141
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 27020:Chemical reaction and process system engineering-related
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | ナノカプセル / ドラッグデリバリーシステム / シリカ / 酵素 / 糖 / 犠牲鋳型 |
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| Outline of Final Research Achievements |
In this study, we have prepared enzyme-encapsulated silica nanoparticles (E@SiO2) through the use of sugar-nanoparticles as templates. The enzymes in the prepared E@SiO2 showed a high activity, comparable to bare one.
Although E@SiO2 have been required in the field of the engineering,it has been hard to achieve to form E@SiO2 without the denaturization of enzyme during the process of preparation. Our simple method will be applied for the wide variety of engineering, especially for drug delivery system.
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| Free Research Field |
反応工学
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| Academic Significance and Societal Importance of the Research Achievements |
中空粒子を調製するために通常，鋳型が必要である．本研究でも鋳型を用いているが，鋳型となる材料に糖のナノ粒子を用いている点が新しい．糖のナノ粒子はこれまでに報告がなく，独自に開発した．水を加えると直ちに溶解して消失するため，中空構造の過渡的な鋳型に利用できる．その結果，あらかじめ酵素を糖ナノ粒子内に埋め込んで，その後，シリカで被覆すれば，水洗浄という極めて温和な条件で鋳型を除去でき，内部に酵素を保持した中空シリカナノカプセルを調製できる．カプセル内の酵素は本来の活性を維持しており，これをドラッグデリバリーシステムへ応用することで，バイオ工学分野の発展が大いに期待される．
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