Development of microbial growth measurement technology for smart cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K05161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Mie University
• Principal Investigator: Miyake HIdeo 三重大学, 生物資源学研究科, 准教授 (50362364)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 熱測定 / 微生物 / 増殖 / スマートセル / 培養

Outline of Final Research Achievements

Material production technology using microorganisms and animal cells has been attracting attention, and the related market is expected to expand rapidly worldwide. However, since microorganisms and animal cell-modified smart cells are produced in large quantities, their analysis is a bottleneck. In particular, culture of these smart cells is essential, and technology to simultaneously monitor the growth process of each individual cell is required. In this study, we developed the smallest cell for measuring microbial growth compatible with shaking culture by improving a microcalorimetry method that can measure the metabolic heat of microorganisms and animal cells with high sensitivity. Furthermore, we have developed a microbial growth measurement technique for smart cells that are equipped with multiple measurement cells.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

ゲノム編集技術などによって様々な優れた機能を有した微生物や細胞（スマートセル）が大量に創出されることが予想される．これらを分析するには培養をする必要があり，大量のサンプルを迅速に測定する技術が求められている．本研究成果は、これら課題を解決できる手法であり，基礎となる基盤技術を確立することに成功した．