2021 Fiscal Year Final Research Report
Development of basic technology for connecting inorganic materials and biomolecules via the biosilica-derived protein
| Project/Area Number |
19K05164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
美藤 友博 鳥取大学, 農学部, 助教 (20776421)
有馬 二朗 鳥取大学, 農学部, 教授 (80393411)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | バイオミネラリゼーション / 海綿動物 / シリカ / 酵素固定化 |
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| Outline of Final Research Achievements |
The present study reveals that glassin, a protein occluded in the silica skeleton of the sponge Euplectella, binds to silica with high specificity at pH 8-9. Detailed analysis demonstrated that the histidine- and aspartic acid-rich region of glassin is responsible for the binding to silica, and that its dissociation constant is 40 nM. In addition to silica, glassin selectively bound to titanium oxide at pH 7-9 and to aluminum oxide at pH 7, but did not show specific binding to titanium oxide and zinc oxide.
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| Free Research Field |
海洋生物工学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果として，グラシンおよびその部分ペプチドは，温和な条件のもと，組換えタンパク質とシリカ，酸化ゲルマニウム，酸化アルミニウムといった無機材料との複合化に有用であることが示された．この技術は，酵素やセンサータンパク質などの工業的に有用なタンパク質のシリカや金属酸化物への固定化，有機分子と無機材料の性質を融合した新規ナノハイブリッド材料やバイオエレクトロニクス材料等の開発に道を拓くと期待される．
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