2021 Fiscal Year Final Research Report
Development of fuctional molecular recognition elements utilizing a highly stable cushioning scaffold protein
| Project/Area Number |
19K05166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 27040:Biofunction and bioprocess engineering-related
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | バイオセンサー / 生体分子間相互作用 / 足場タンパク質 / 表面親和性ペプチド / 固定化 / 分子認識素子 |
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| Outline of Final Research Achievements |
In order to establish a simple and rapid biosensing system for detection of biomolecular interactions on solid substrates, we attempted to expand the molecular design diversity of CutA1, a novel scaffold protein, and verified its function. An objective multivalent CutA1 was successfully developed by identifying a novel sequence insertion site. In addition, single-chained CutA1, for another type of multivalent molecular recognition element, was also constructed by the insertion of appropriate linker sequences between CutA1 subunits. As for the detection of biomolecular interactions, the detection sensitivity can be improved by controlling the arrangement and orientation of the ligand molecules and by introducing irregularities at the nanobio-interface.
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| Free Research Field |
蛋白質工学
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| Academic Significance and Societal Importance of the Research Achievements |
バイオセンサーへの適用に向け，簡便な調製が可能で，構造が極めて安定な超好熱菌由来タンパク質CutA1を用い，標的分子認識素子（ペプチドあるいはタンパク質）を提示するための足場として利用する新しいバイオ分子間相互作用制御システムの技術基盤を確立した．本研究の成果により，独自のアイデアに基づき，医療（診断）・創薬・食品・環境分野といった幅広い分野で適用可能な，多様で高感度なバイオセンサーの開発が期待できる．
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