Development of scaffolds for oriented immobilization of biosensing molecules and their application to biosensing technology

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K05219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 28030:Nanomaterials-related
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: IIJIMA Masumi 東京農業大学, 応用生物科学部, 教授 (40390728)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: ナノバイオ / ナノ材料 / 生体分子 / 整列化 / 足場分子

Outline of Final Research Achievements

We have developed a bio-nanocapsule (BNC) scaffolds that clusters and oriented immobilization of IgG and Fc fusion receptors, enabling higher sensitivity and lower cost in various biosensensors. However, there were some issues, such as insufficient effect of increased sensitivity. In this study, we aimed to improve the BNC scaffold technologies and develop new scaffolds with evolved BNC characteristics to develop a technology that enables simple and ultra-sensitive detection of small amounts of substances to be measured. As a result of this study, we succeeded in developing a simple and highly sensitive detection of food allergens using the BNC scaffolds. In addition, a new scaffold, DNA aptamer orientation-presenting BNC, was developed to achieve ultra-sensitive detection of ligands.

Free Research Field

ナノバイオテクノロジー

Academic Significance and Societal Importance of the Research Achievements

本研究成果のBNC足場技術によるイムノスティック法の簡便・高感度検出、および新規足場分子DNA整列提示型BNCを用いたDNAアプタマーの整列化およびリガンド検出の高感度化は、他に類を見ない技術であり、その独自性や優位性は非常に高いと考える。今後、本技術は食品製造工場や医療現場などにおける、様々な抗原の簡易測定キットなどへの応用も期待できることから、その学術的意義や社会的意義は高いと考える。