2021 Fiscal Year Final Research Report
Development of highly-biocompatible super-resolution imaging based on fluorescence polarization and fluctuation
| Project/Area Number |
19K05226
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 28040:Nanobioscience-related
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 超解像イメージング / 蛍光顕微鏡 / 光スイッチング蛍光タンパク質 / 蛍光偏光 / cumulant |
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| Outline of Final Research Achievements |
In conventional super-resolution imaging involving fluorescence microscopy, a sample is needed to be irradiated at a considerably high power density, which leads to a serious problem of phototoxicity in cells especially when observing a live sample. To largely mitigate the phototoxicity, we recently developed a super-resolution imaging technique SPoD-OnSPAN. However, because fluorescent objects to be observed by SPoD-OnSPAN in principle need to exhibit fluorescence polarization, high resolution observation of a fluorescent object which contains many fluorescent probes with various orientations has been difficult. To solve this problem, this study has newly developed a super-resolution imaging technique that combines SPoD-OnSPAN and higher-order statistics of fluorescence fluctuation.
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| Free Research Field |
生物物理学
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| Academic Significance and Societal Importance of the Research Achievements |
SPoD-OnSPAN超解像顕微鏡は、STED、RESOLFT, SIMといった高度で特化した顕微鏡装置が必要な手法とは異なり、かなりシンプルな光学系を有している。しかし、従来のSPoD-OnSPANでは、蛍光異方性が低い蛍光物体の高空間分解能化が難しいこと、そして画像再構成にかなり長い計算時間を要した。ところが、本研究課題で開発した手法では、これらの問題を解決していると思われる。今後、本研究で得られた成果をさらに発展させることにより、超解像イメージングがより一層広く生命科学研究で利用されることに本研究の成果が資するものと期待する。
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