2021 Fiscal Year Final Research Report
Nanometer scale resolution imaging of pH and reactive oxygen species on the outer membrane surface for fast and effective cell discrimination in cancer tissues.
| Project/Area Number |
19K05228
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 28040:Nanobioscience-related
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山田 忠明 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00507048)
細木 誠之 京都薬科大学, 薬学部, 准教授 (30433254)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | Raman spectroscopy / Nanosensors / pH / Hydrogen peroxide / cell surface |
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| Outline of Final Research Achievements |
We developed and tested a nano-sensor for quantification of hydrogen peroxide (H2O2) on the cell surface. The nanosensor is based on bioconjugated gold nanoparticles that can be anchored to the extracellular cell surface and can quantify H2O2 using surface enhanced Raman spectroscopy (SERS). We successfully tested the new nanosensor on adenocarcinomic human alveolar basal epithelial cells (A59 cell line) for highly localized quantitative measurements of H2O2. In addition, we optimized the use of our previously developed pH nanosensor for cell surface pH imaging. Using a laser line illumination mode, we simultaneously acquired 400 SERS spectra from a wide area of the cell sample. We analyzed different cell lines: pancreatic cancer cell BxPC; pancreatic cancer cells AsPC-1; murine fibroblast cells NIH/3T3; human fibroblast WI-38; lung cancer A549.
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| Free Research Field |
Biophysics
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| Academic Significance and Societal Importance of the Research Achievements |
Quantification of pH and hydrogen peroxide on the cell surface is critically important to study many cellular functions and can be exploited to discriminate cancer cells from normal cells in tissues. The nanosensors developed in our research project showed great potential to accomplish these goals.
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