2021 Fiscal Year Final Research Report
Uranyl binding peptide for nanopore sequencing
Project/Area Number |
19K05325
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 31010:Nuclear engineering-related
|
Research Institution | Tokyo Institute of Technology |
Principal Investigator |
Tsushima Satoru 東京工業大学, 科学技術創成研究院, 特任准教授 (80312990)
|
Co-Investigator(Kenkyū-buntansha) |
鷹尾 康一朗 東京工業大学, 科学技術創成研究院, 准教授 (00431990)
|
Project Period (FY) |
2019-04-01 – 2022-03-31
|
Keywords | ウラニルイオン / ペプチド |
Outline of Final Research Achievements |
Cyclic peptides as well as a modified EF-hand motif of calmodulin have been newly designed to achieve high affinity towards uranyl(VI). Cyclic peptides may be engineered to bind uranyl(VI) to its backbone under acidic conditions, which may enhance its selectivity. For the modified EF-hand motif of calmodulin, strong electrostatic interactions between uranyl(VI) and negatively charged side chains play an important role in achieving high affinity; however, it is also essential to have a secondary structure element and formation of hydrophobic cores in the metal-bound state of the peptide.
|
Free Research Field |
アクチノイド化学
|
Academic Significance and Societal Importance of the Research Achievements |
本研究により、ウラニルイオンに選択的なペプチドを新たに開発することができた。この成果によりウランとペプチドの分子レベルでの相互作用を系統的に明らかにすることができた上、ウランと生体物質の相互作用の礎となる知識を新たに蓄積できた。この知見を学界で広く共有することにより、この分野の新たな展開と発展への学術的貢献が実現できると考える。
|