2022 Fiscal Year Final Research Report
The development of light-sensitive antibody for controling protein activities.
Project/Area Number |
19K05538
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 34020:Analytical chemistry-related
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Research Institution | The University of Tokyo |
Principal Investigator |
Endo Mizuki 東京大学, 大学院理学系研究科(理学部), 助教 (80793231)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | 光制御 / 抗体 / 金属ナノ粒子 |
Outline of Final Research Achievements |
In the present project, we aimed to develop a light-sensitive antibody for controlling endogenous protein activities, and establish a novel analytical platform with the help of unique metal nanoparticles. As for the light-sensitive antibody, we succeeded in the development of the molecule that controls endogenous protein activities reversibly or irreversibly. On the other hand, we found a novel metal nanocluster that retains phosphorescence in the aqueous solution. The route for cellular uptake of the nanocluster was modulated by the ligand molecules surrounding the metal cores. Utilizing the long lifetime of phosphorescence, the incorporated nanocluster was successfully visualized with phosphorescence lifetime imaging with high contrast, where background autofluorescence signal is sufficiently minimized.
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Free Research Field |
分析化学
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Academic Significance and Societal Importance of the Research Achievements |
開発した光機能性抗体を用いることで,内在性タンパク質の活性を可逆的・不可逆的に人為的に制御することが可能となり,時空間的に変動するタンパク質活性パターンの生理的意義の解明につながると期待される.また,開発した金属ナノクラスターは、そのリン光発光を利用した細胞イメージングが可能となり,細胞内の取込み経路や集積させる細胞小器官を自在に制御できる可能性を示すことができた.本研究成果は,多元素金属ナノクラスターの化学を拓き、光バイオ分析のための新たな物質群と基礎技術を提供することが期待される.
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