2021 Fiscal Year Final Research Report
Search for biological active compounds based on culture methods through communication between two microorganisms
| Project/Area Number |
19K05709
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
|
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2022-03-31
|
| Keywords | 共培養 / コクリオキノン / 細胞毒性 / Clonostachys rosea / Nectria pseudotrichia / Arthrinium marii |
|---|
| Outline of Final Research Achievements |
Three new meroterpenoid derivatives, furanocochlioqiunol (1) and furanocochlioqiunone (2), as well as nectrianolin D (3), together with two known biogenetically related compounds 4 and 5 were isolated from a mixed culture of two mangrove-derived fungi, Clonostachys rosea B5-2 and Nectria pseudotrichia B69-1. The structures of 1-3 were deduced based on the interpretation of HRMS and NMR data. Compounds 1-5 exhibited cytotoxicity against human promyelocytic leukemia (HL60) cells with IC50 values ranging from 0.47 to 10.16 μM. A novel 2,3-epoxy naphthoquinol, named (6R,7R,8R)-theissenone A (1), possessing an oxatricyclo[5.4.0.03,5]undeca-trien-2-one skeleton, together with two known compounds, (6S,7R,8R)-theissenone (2) and arthrinone (3), were produced by an endophytic fungus, Arthrinium marii M-211.
|
| Free Research Field |
天然物有機化学
|
| Academic Significance and Societal Importance of the Research Achievements |
これまでに天然有機化合物の単離精製に関する研究は多くなされてきたことから、新規な二次代謝産物を得ることは簡単ではないと言われている。その一方、放線菌や糸状菌のような有用な物質を生産する酵素の遺伝情報が明らかにされると、予想以上に多くの生合成遺伝子クラスターを持っていることが判明した。共培養法により、微生物細胞間でのコミュニケーションや細胞間の直接の接触刺激による物質生産における制御機構を明らかにできれば、二次代謝産物の生産を活性化させる菌類の組み合わせが判明すると共に、これまで以上に、共培養法により新規骨格を有する生理活性物質の発見に期待できる。
|
