Development of peptide nucleic acids recognizing double-stranded DNA with high efficiency

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05730
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 37030:Chemical biology-related
• Research Institution: Nagoya University
• Principal Investigator: Aiba Yuichiro 名古屋大学, 理学研究科, 准教授 (10581085)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: DNA / PNA / ペプチド核酸 / インベージョン / 核酸 / 遺伝子

Outline of Final Research Achievements

Sequence-selective recognition of double-stranded DNA in cells will enable the control of gene expression and associated biological functions. Therefore, it is expected to be applied to a wide range of research fields, including the elucidation of various biological processes and the therapeutic treatment of genetic diseases. A new method for sequence-selective recognition of double-stranded DNA has been expected to be developed as a fundamental technology in nucleic acid research because of its high applicability. In this study, we constructed a new double-stranded DNA recognition (invaision) technology using peptide nucleic acid (PNA), a type of artificial nucleic acid.

Free Research Field

核酸化学

Academic Significance and Societal Importance of the Research Achievements

本研究において開発したペプチド核酸（PNA）による2本鎖DNA認識技術は、従来高いDNA認識効率を達成するうえで必要不可欠であったPNAへの化学修飾を不要とし、より簡便なPNA合成を達成している。これにより、付随する様々な障壁を取り払うことで、PNAによる2本鎖DNA認識手法（インベージョン）がより応用研究に適応しやすくなり、その汎用化が期待される。