Molecular ecological analysis on rhizosphere microbial community responding to above-ground light environment

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05759
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38010:Plant nutrition and soil science-related
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Ikeda Seishi 国立研究開発法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 上級研究員 (20396609)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: イネ / Tos17 / 共生微生物 / 光 / フィトクローム / 多様性解析 / 葉 / 根

Outline of Final Research Achievements

Bacterial and fungal community structures in roots of rice (cv. Nipponbare) were compared between the wild type and phytochrome-associated Tos17 mutants. As a result, the relative abundances of 5 bacterial groups at phylum level (FCPU426, Fibrobacteres, Nitrospirae, Spirochaetes, WS2) and 4 fungal groups at class level (Dothideomycetes, Saccharomycetes, Sordariomycetes, Ustilaginomycetes) in roots of mutants were significantly different relative to those in wild type. In addition, community analysis of leaf-associated fungi at class level revealed that the relative abundance of Microbotryomycetes in a mutant was also significantly different relative to that in wild type rice.

Free Research Field

植物共生微生物学

Academic Significance and Societal Importance of the Research Achievements

光受容体に関係する遺伝子変異がイネの根や葉の共生微生物群に大きな影響を与えることが明らかにされたことから、植物の光情報伝達関連の生理生態が植物共生微生物について高次の分類群レベルで動態や生態に強い影響を及ぼしていることが示唆された。それらの知見に基づいた光情報伝達系や光環境の制御を介した植物共生微生物の効果的な利用・制御、というような新規の農業微生物制御技術の開発としての可能性が期待できる。