Systematization of microbial bio-vinyl compounds and its application as seed compounds for drug development

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05767
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Aso Yuji 京都工芸繊維大学, 繊維学系, 教授 (70380590)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: イタコン酸類縁体 / 発酵生産 / 創薬 / ポリマー合成

Outline of Final Research Achievements

A method “DISCOVER” for screening of microbials producing biovinyl compounds based on thiol-ene reaction and Heck reaction was developed. Aspergillus niger S17-5 producing 9-, 10-hydroxyhexylitaconic acids was isolated from a soil using the method. Production of these compounds was enhanced by adding octanoic acid, suggesting that these compounds are synthesized via alkylcitric acid cycle. Especially, 9-hydroxyhexylitaconic acid showed a cytotoxicity against cancer cells HeLa. Copolymers consisting of 10-hydroxyhexylitaconic acid and itaconic acid were synthesized by free radical polymerization. The molecular weight of the resulting polymers was decreased with increase of the number of 10-hydroxyhexylitaconic acid unit.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は応用微生物学・創薬化学・高分子合成化学を融合する先駆的な試みである。特に、申請者が開発した技術以外にバイオビニル生産菌を選択的に分離する方法はなく、バイオビニルを創薬原料として利用する試みもないため、本研究は優位性と独創性に優れる。また、新規遺伝子・酵素の取得や新規バイオプロセス法の開発（応用微生物学）、新薬の開発や新規予防医療技術の提供（創薬化学）、新規重合法や新規物性ポリマーの開発（高分子合成化学）など、幅広い学術領域への波及効果が期待できる。