2021 Fiscal Year Final Research Report
Development of chemoenzymatic synthesis of amino acids and derivatives based on enzyme engineering
| Project/Area Number |
19K05775
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Ritsumeikan University (2020-2021)
Toyama Prefectural University (2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平田 佳之 大阪医科薬科大学, 薬学部, 助教 (00745854)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | アミノ酸酸化酵素 / ケト酸 / アミド化合物 |
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| Outline of Final Research Achievements |
Synthesis of unnatural amino acids and peptides is an important technology in the field of drug discovery, especially for multi-product synthesis and mass production. In this study, keto acids, which are difficult to synthesize organically, were synthesized from amino acids using enzymes, followed by the condensation of keto acids with hydroxylamine derivatives in the absence of chemical reactants to produce amino acids and peptides. This technology incorporates elements of parallel synthesis for synthesizing multiple products and flow synthesis that can be used for mass production using immobilized enzymes, and could be a useful synthetic technology for mass production of various amino acid and peptide syntheses.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
1. ケモエンザイマティック反応による非天然アミノ酸や特殊ペプチド合成:有機合成ではなくアミノ酸酸化酵素を用いた酵素反応で、迅速(一段階反応)・簡便(保護や脱保護が不必要)・有毒な試薬を使用せず合成するケモエンザイマティック反応自体に新規性があり、今後のペプチド医薬品開発に貢献できた。
2. 酵素学の観点からのケモエンザイマティック反応の改良:本研究では新技術を確立した後に、自然界から得た酵素や変異導入酵素をあてはめ、ケモエンザイマティック反応で新しい酵素反応を探索した。これにより従来の酵素探索から応用までの間に起こりうる問題を回避することができ、産業用酵素の開発の時間短縮につながった。
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