Characterization of enzymes involved in C-glycosides metabolism

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05784
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: University of Tsukuba
• Principal Investigator: Kumano Takuto 筑波大学, 生命環境系, 助教 (70585025)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: C-配糖体 / 微生物

Outline of Final Research Achievements

In nature, there are various glycosides produced by plants and other organisms, and some of these compounds have attracted attention for their bioactivity. While most glycosides are O-glycosides, in which a sugar is attached to an oxygen atom, the compounds in this study are C-glycosides, in which a sugar is attached directly to carbon of aglycone. C-glycosides are more stable against heat, acid, and enzymes than O-glycosides. In this study, we discovered enzymes that can efficiently degrade C-glycosides from microorganisms that catabolize C-glycosides, and succeeded in clarifying their detailed biochemical properties as well as their three-dimensional structures. The results of this research are expected to contribute to the further development of research on C-glycoside metabolism in the future.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

C-配糖体が他のO-配糖体とは全く異なる経路で代謝されることを解明し、その反応に関わる２種の酵素を同定した。さらに本研究により世界で初めて微生物由来のC-配糖体代謝酵素２種の結晶構造解析が明らかになった。結晶構造の解明により、反応を触媒するアミノ酸が特定され詳細な反応機構を提案することができた。C-配糖体を代謝する酵素が明らかになったことでそれらの酵素を利用し、分解されにくいC-配糖体を分解することで新たな生理活性物質の創出にも繋がる可能性がある。