2021 Fiscal Year Final Research Report
Research on switching of mRNAs targeted by sRNA and regulation of sRNA stability in bacteria
Project/Area Number |
19K05787
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
|
Research Institution | Niigata University |
Principal Investigator |
|
Project Period (FY) |
2019-04-01 – 2022-03-31
|
Keywords | 遺伝子発現 / small RNA / Csrシステム / RNA結合タンパク質 / 応用微生物学 / キチナーゼ |
Outline of Final Research Achievements |
In bacteria, the antisense sRNA ChiX, which regulates the expression of the chitinase genes, switched the target mRNA from one to the other. In this process, base pairing between the terminator region of the sRNA and the mRNA was important. In the bacterial Csr system, the critical amino acid residue of CsrD that regulates the stability of protein-bound sRNA was identified. In addition, the activity of CsrD responded quickly to the external environment. The possibility of using this mechanism to produce polysaccharides, a component of bacterial biofilms, was demonstrated.
|
Free Research Field |
応用微生物学
|
Academic Significance and Societal Importance of the Research Achievements |
本研究により、細菌のsRNAによる遺伝子発現調節機構における標的RNAの切り替えとsRNAの分解制御機構が明らかになる。この研究成果は、近年注目されているsRNAにおけるアンチセンス型とタンパク質結合型sRNAの新たな機能の理解や分解制御機構の解明に貢献するだけでなく、細菌の新たな生命現象の解明につながる。さらに、sRNAを利用した細菌による新たな物質生産方法開発などの応用に役立つと考えられる。
|