Elucidation of mechanisms for high-temperature resistance of super thermotolerant wild yeasts and its application to fermentation biotechnology

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05790
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Hiroshima Institute of Technology (2021); Osaka University (2019-2020)
• Principal Investigator: SUGIYAMA Minetaka 広島工業大学, 生命学部, 教授 (80379130)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 出芽酵母 / 高温耐性 / バイオエタノール / 発酵生産

Outline of Final Research Achievements

To study the thermotolerant mechanism of yeast for biotechnological applications, a Saccharomyces cerevisiae wild strain isolated from nature, which shows an excellent thermotolerance at up to 42℃, was analyzed. Results showed that enhanced ribosome biogenesis, robust cell cycle machinery and up-regulated expression of anti-oxidant genes based on the higher induction of SFP1 and GCN4 genes involved in ribosome biogenesis and amino acid metabolism, respectively, played important roles to confer superior thermotolerance on the yeast. Since thermotolerance of yeast is one of the most important characters for cost-effective fermentative production in tropical countries where biomass energy is much abundant, these findings will contribute to the improvement of microbial production that can help to achieve SDGs.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

近年の地球温暖化問題によって、発酵生産に使用する微生物の高温耐性化が急務となっている。本研究では、発酵生産において中心的な役割を果たす出芽酵母の高温適応機構の一端を明らかにし、高温条件下でのバイオエタノールや乳酸の高生産化に成功したことから、SGDsの達成に重要となる発酵生産の効率化に貢献することが期待される。