Construction of the brown macroalgae-based biorefinery system for the high production of terpenoid and lipids

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05796
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Ishikawa Prefectural University
• Principal Investigator: Kawai Shigeyuki 石川県立大学, 生物資源環境学部, 教授 (00303909)
• Co-Investigator(Kenkyū-buntansha): 橋本 渉 京都大学, 農学研究科, 教授 (30273519) ; 三沢 典彦 石川県立大学, 生物資源環境学部, 教授 (30393466)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 酵母 / テルペノイド / 褐藻 / 海藻 / アルギン酸 / 油脂 / アルギン酸リアーゼ / DEH

Outline of Final Research Achievements

In this study, the plasmid for the expression of ylacs1, ylHmg1, and ylAcl1 was constructed to improve the biosynthetic pathway of terpenoid in Yarrowia lipolytica. Moreover, the capacity to utilize DEH was conferred on Y. lipolytica, resulting YliDEH+ strain. The condition to produce DEH from sodium alginate was optimized e.g. through optimizing the amounts of endo- and exo-type alginate lyases. Lipids were produced from each glucose and DEH by using the YliDEH+ strain and analyzed by GC-MS. Composition of the lipid from DEH was similar to that from glucose. The amount of the former was lower than that of the latter.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

海藻（褐藻）資源は、広大な管轄水域を有する日本で豊富に得られる有望な資源であが、その利用には微生物への褐藻主要成分アルギン酸（DEH）資化能の付与等による、海藻（褐藻）バイオリファイナリー技術（微生物の利活用により褐藻から有用成分を生産する技術）の開発が鍵となる。本研究では、はじめて海藻資源（アルギン酸由来のDEH）からの油脂の生産に成功し、持続可能な新しい同技術開発の重要な基盤となる成果を得た。