Construction of a resource library of actinomycete promoters

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K05806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Jun Ishikawa 国立感染症研究所, 品質管理研究センター, 主任研究官 (40202957)
• Co-Investigator(Kenkyū-buntansha): 星野 泰隆 国立感染症研究所, 真菌部, 主任研究官 (40399457)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 放線菌 / プロモーター

Outline of Final Research Achievements

After careful examination of the high-quality Transcriptional Start Site (TSS) obtained in KAKENHI No. 26430201, 3,220 upstream sequences of TSSs where candidate promoter sequences were obtained. We explored the distribution of 6-mer in those sequences, and found 88 6-mers were found. Next, as a result of analyzing the position and number of occurrences of each 6-mer, we found 6-mer (sequences characteristic of promoters) that occur at high frequency around -12 and -40 nucleotides upstream of TSS. By searching upstream sequences containing both 6-mer that appear around -12 and -40 nucleotides, 31 candidate promoter sequences were found.

Free Research Field

微生物ゲノム

Academic Significance and Societal Importance of the Research Achievements

多剤耐性菌の問題の深刻化など、新たな抗生物質の登場が切望されているが、既存抗生物質の誘導体ではない真に新しい抗生物質を開発するために、ゲノム情報を用いた開発が行われている。しかしながら、その道具立ては十分とは言えず、さらに、放線菌のプロモーターに関する網羅的な研究は数例しかないため、遺伝子発現を自在に操作し得る多様なプロモーターを含むリソースの提供は、抗生物質開発などの放線菌ゲノム工学ばかりでなく、放線菌の学術的理解に資すると考えられる。