Study on molecular regulatory mechanisms in thermo-sensitive male sterility of rice based on cell-free translation system

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: Saitama University
• Principal Investigator: Tozawa Yuzuru 埼玉大学, 理工学研究科, 教授 (90363267)
• Co-Investigator(Kenkyū-buntansha): 川岸 万紀子 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (50355707)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 転写因子 / イネ / 高温不稔

Outline of Final Research Achievements

The rapid global warming has a great impact on the production of major cereals such as rice, and the high-temperature conditions during the flowering season not only deteriorate the quality of seeds but also sometimes cause sterility. This problem of high-temperature sterility is one of the important issues to be solved in order to maintain food production in the future. The principal investigators predicted transcription factors that it might be involved in high-temperature male sterility in rice. To conduct a functional analysis of these transcription factors at the protein level, the candidates were synthesized and purified as a full-length protein by using a cell-free translation system based on the wheat germ extract. It has been confirmed that one transcription factor, OsMyb80, shows specific binding to the regulatory region of the gene CYP703, which is essential for pollen formation.

Free Research Field

タンパク質科学、分子生物学

Academic Significance and Societal Importance of the Research Achievements

高温雄性不稔に関連する転写因子のタンパク質レベルでの機能解析系の構築は、温度感受性を生化学的に検証する上で重要な技術的プラットホームの提供につながると期待される。高温条件で影響を受けやすい分子機構の解明を加速することにより、タンパク質工学やゲノム編集を利用した転写制御様式のオーダーメイド型改変など、イネなどの主要作物の高温条件対策を具体的に進める応用研究の下地になるものと考えられる。