Analysis of yeast TRPY1 channel which convert physical stimulations into biochemical signals.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K05823
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38030:Applied biochemistry-related
• Research Institution: The University of Tokyo
• Principal Investigator: Hamamoto Shin 東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (10533812)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: イオン輸送 / チャネル / オルガネラ / 出芽酵母

Outline of Final Research Achievements

Saccharomyces cerevisiae possesses a transient receptor potential (TRP) channel homolog TRPY1 in its vacuolar membrane. So far, studies have focused on the channel properties of TRPY1, but its regulation and physiological role remained to be elucidated. TRPY1 can be activated by hyperosmotic shock in vivo and release Ca2+ from the vacuole into the cytolplasm, which can be measured by expressing aequorin in cytoplasm. To gain further observation of TRPY1 at a molecular level, we screened the collection of yeast single gene deletants for alterations in hyperosmotic response in an attempt to find factor that regulates the activity of TRPY1. We have identified several deletion mutant strains which shows decreased response against to hyperosmotic condition. Some of the deleted gene encoded organelle membrane protein and suggested a novel mechanism to activate TRPY1.

Free Research Field

イオン輸送

Academic Significance and Societal Importance of the Research Achievements

本研究における成果は、他の生体膜と比較して研究が遅れているオルガネラ膜に局在性を示すイオンチャネルの活性制御機構の一端を明らかにしたことである。出芽酵母は、発酵生産環境では高浸透圧ストレスに晒されているため、高浸透圧ストレスで活性化するTRPY1の研究で得られた知見の出芽酵母の発酵生産環境への応用が期待される。さらに、TRPY1と類似のイオンチャネルは広く高等生物に保存されており、多くの疾病をも関わっていることが知られているため、これらの疾病の原因解明へのが期待される。