2021 Fiscal Year Final Research Report
Screening of novel carbohydrate phosphorylase using carbohydrate assimilation modified Escherichia coli
Project/Area Number |
19K05824
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38030:Applied biochemistry-related
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Research Institution | Niigata University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | ホスホリラーゼ |
Outline of Final Research Achievements |
Phosphorylases are exolytic enzymes catalyzing phosphorolysis of particular glycosides to produce sugar 1-phosphate with strict substrate specificity. The reaction is reversible, enabling the practical synthesis of oligosaccharides. However, there is little variation among phosphorylases and this limits their utilization for the production of oligosaccharides. Therefore, it would be beneficial to identify phosphorylases with previously unreported substrate specificities. In this study, we performed screening of novel enzymes by bioinformatics method and metagenomics method specialized for novel phosphorylase using carbohydrate assimilation modified Escherichia coli.
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Free Research Field |
糖質科学
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Academic Significance and Societal Importance of the Research Achievements |
新規ホスホリラーゼの探索は、新たな生体内糖代謝機構の提唱に繋がり、生物化学的意義から学術的に大変重要な研究である。さらに新規酵素群を活用したオリゴ糖高収率合成法の確立により、特性及び機能評価が未実施だった様々なオリゴ糖がライブラリー化され、且つ大量調製可能となり、様々な学術研究へのオリゴ糖供給が可能となる。また、オリゴ糖の網羅的機能性評価の実施により、これまで明らかにされてこなかったオリゴ糖の生理機能と構造の相関が解明されることで、今後特定の機能性を追求したオリゴ糖の選択・利用が可能になり、機能性オリゴ糖の食品及び医薬品素材として応用性を見出すことで、食品・医療産業界へ貢献を果たす。
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