2021 Fiscal Year Final Research Report
Elucidation of mechanisms of insecticidal actions of two-domain scorpion peptides
| Project/Area Number |
19K05842
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38040:Bioorganic chemistry-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | サソリ毒 / ペプチド / 殺虫活性 / 抗菌活性 / 生体膜 |
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| Outline of Final Research Achievements |
In scorpion venom, there are two types of peptides as active components: peptides with disulfide bonds and peptides that form an alpha-helix structure without disulfide bonds. However, there are also two-domain peptides with both structural features, which are called beta-KTx peptides. These peptides have been found in venoms of many scorpion species, but the role of each domain structure in the expression of biological activity remains unclear. Therefore, we identified and synthesized LaIT3 and LaIT4, beta-KTx peptides found in Liocheles australasiae scorpion venom. As a result, both peptides were obtained, using the native chemical ligation method in high purity. This allows efficient synthesis of these peptides with the amount required for elucidation of their mechanism of action.
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| Free Research Field |
ペプチド化学
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| Academic Significance and Societal Importance of the Research Achievements |
サソリ毒液には様々な生理活性をもつペプチド成分が数多く含まれ、農薬あるいは医薬品としての応用が期待されている。しかし、その成分の一種である２ドメイン型ペプチドについてはその構造と活性の関係において不明な点が多い。同様のペプチドは、クモ毒からも見いだされていることから、生物毒素がこのような構造を形成することに利点があるものと推察される。本研究において、２ドメイン型ペプチドの化学合成法を確立できたことから、その作用メカニズムが進むと考えられ、今後の農薬・医薬品開発において有用な情報を与えるものである。
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