Study for reaction control of multifunctional cytochrome P450 enzyme MycG

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K05851
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38040:Bioorganic chemistry-related
• Research Institution: Toho University
• Principal Investigator: Anzai Yojiro 東邦大学, 薬学部, 教授 (20318299)
• Co-Investigator(Kenkyū-buntansha): 福本 敦 東邦大学, 薬学部, 講師 (50516391) ; 飯坂 洋平 東邦大学, 薬学部, 講師 (40770425)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 多機能型酵素 / チトクロームP450酵素 / マクロライド系抗生物質 / 生合成 / 反応制御

Outline of Final Research Achievements

We attempted to regulate the reaction of the multifunctional cytochrome P450 enzyme MycG, which is involved in several kinds of oxidation reactions in the biosynthesis of 16-membered ring macrolide antibiotic mycinamicin produced by Micromonospora griseorubida A11725. Three MycG mutants in which epoxidation of the biosynthetic intermediate M-V is retarded were obtained from the MycG random mutant library. M. griseorubida TPMA0075, in which the gene encoding the mycG mutant V135G/E355K was introduced into the mycG gene disruptant strain M. griseorubida TPMA0025, produced 10 to 40 times more amount of M-V than the wild strain M. griseorubida A11725 or the control strain M. griseorubida TPMA0047 (M. griseorubida TPMA0025 + mycG).

Free Research Field

微生物化学

Academic Significance and Societal Importance of the Research Achievements

複数種類の触媒反応を示す多機能型酵素の部分的な触媒反応の制御は、酵素反応や発酵における目的化合物や稀少化合物の効率的な取得や生産に繋がる。