The effect of flavonoid from the mucosal side in intestinal organoids

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K05925
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38050:Food sciences-related
• Research Institution: Yamagata University
• Principal Investigator: Kozakai Takaharu 山形大学, 地域教育文化学部・理工学研究科, 教授 (70391480)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 腸管上皮 / 化学受容認識 / 能動輸送 / 経上皮短絡電流 / オルガノイド

Outline of Final Research Achievements

To investigate the function of flavonoids, the effects of absorption capacity of nutrients such as glucose in the intestinal tract, we aimed to develop a novel method using an organoid cell culture system while comparing it with the conventional Ussing-chamber tissue culture method. Organoids were formed from small intestinal tissue of adult mice under isolation conditions that contained approximately twice as many stem cell marker LGR5-positive cells. However, satisfactory experimental operability was not achieved. In tissue culture, proanthocyanins inhibited Na+ flax co-transported with glucose. On the other hand, bile acids promoted active ion transport in the small intestine but inhibited it in the large intestine. It is necessary to study the Na+-dependent absorption in each nutrition and/or the chemical substance.

Free Research Field

栄養生理学

Academic Significance and Societal Importance of the Research Achievements

オルガノイド細胞培養法を改良して栄養素の吸収能を精密に計測しようと試みた。オルガノイドのハンドリングなど十分な実験操作性が得ることができなかったが、オルガノイド の供試した新規実験法の基盤的知見を得ることができた。また、フラボノイドなど、摂食する食成分やそれに対応した分泌成分などの影響を受けることを解明した。これらの結果から、腸管部位ごとのオルガノイド培養系による吸収評価法が必要であることが理解できた。食品栄養成分の腸管における吸収能力をより精密に理解して、ヒトの精密栄養管理技術に貢献する基礎成果を得ることができた。