2021 Fiscal Year Final Research Report
Regulatory mechanisms of chromatin dynamics involving an interplay between HMG proteins and histones
| Project/Area Number |
19K05957
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38060:Applied molecular and cellular biology-related
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| Research Institution | Meisei University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | ヌクレオソーム / ヒストン / HMGBタンパク質 / クロマチン動態制御 / ゲノム機能発現 / 出芽酵母 / ケミカルマッピング / タンパク質-DNA相互作用 |
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| Outline of Final Research Achievements |
The nucleosome is the fundamental unit of eukaryotic chromatin, and its structure dynamically changes during gene transcription, DNA replication and repair processes. The aim of this research is to clarify the mechanism of nucleosome dynamics by uncovering the interplay between nucleosomes and non-histone proteins. In the present study, we developed a novel method to analyze and nucleosome positions, and DNA binding sites of a non-histone protein Hmo1 (yeast HMG homolog) in the yeast genome. We suggested a model in which Rap1 recruits Hmo1 to deplete nucleosomes at the promoter region during transcriptional activation. Furthermore, we established a genome-wide mapping method of nucleosome positions using next-generation sequencing. These techniques are also useful for examining the effects of DNA sequences on nucleosome formation in vivo.
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| Free Research Field |
分子生物学、生化学、分子遺伝学
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| Academic Significance and Societal Importance of the Research Achievements |
クロマチンの基盤ユニットであるヌクレオソームは、DNAの塩基配列変化を伴わない遺伝子発現制御機構であるエピジェネティクスのプラットフォームである。本研究において、細胞内でのヌクレオソームと非ヒストンタンパク質のDNA結合部位の新規解析法を確立し、遺伝子の転写制御におけるヌクレオソームと非ヒストンタンパク質の動態変化を明らかにした。本研究で確立したクロマチン構造の新規解析法はエピジェネティック制御機構の解明と、その破綻による疾患研究の新機軸の開拓へ繋がることが期待される。
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