Analysis of genes responsible for preharvest sprouting in Brassicaceae

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05969
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Mie University
• Principal Investigator: Kagaya Yasuaki 三重大学, 地域イノベーション推進機構, 准教授 (20335152)
• Co-Investigator(Kenkyū-buntansha): 諏訪部 圭太 三重大学, 地域イノベーション学研究科, 教授 (50451612)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 穂発芽 / 休眠 / アブシシン酸 / オーキシン / 転写 / アブラナ科野菜

Outline of Final Research Achievements

In Japan, because seeds of Brassicaceae are harvested during the rainy season, high frequency of preharvest sprouting is a serious problem in the seed industry. In this study, we performed genetic and molecular analyses for preharvest sprouting to elucidate the responsible genes in Brassicaceae. Genomic, gene expression, and promoter expression analyses revealed the conserved mutations in the promoters of the embryo growth arrest regulator IAA30 in Brassicaceae. These mutations were suggested to be responsible for the high incidence of preharvest sprouting in Brassicaceae. Furthermore, we found that preharvest sprouting in Brassicaceae is caused by maternal effects regulated via multiple QTLs.

Free Research Field

植物分子遺伝学

Academic Significance and Societal Importance of the Research Achievements

アブラナ科野菜の穂発芽の研究はこれまでほとんど行われていない。本研究では、アブラナ科野菜に共通して存在する胚成長停止制御因子の普遍的な変異と母性効果で作用する複数のQTLを見出した。本研究は、双子葉植物での種子休眠の確立と穂発芽の発生原因における新知見を提供するとともに、穂発芽を防ぐための新採種技術確立のための情報基盤として波及できる。