Understanding of molecular mechanisms to destine to form a constitutive barrier to radial oxygen loss in wild rice species

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K05978
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39010:Science in plant genetics and breeding-related
• Research Institution: Fukui Prefectural University
• Principal Investigator: Shiono Katsuhiro 福井県立大学, 生物資源学部, 准教授 (20610695)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 湿害 / 洪水 / 低酸素 / 野生イネ / 気候変動

Outline of Final Research Achievements

A barrier against radial oxygen loss (ROL) is a crucial trait for waterlogging tolerance in wetland plants. Rice inducibly forms the ROL barrier under waterlogged conditions. Therefore, rice has been used as a model to understand the molecular mechanisms of the barrier formation mechanism. But, it has been not identified upstream key genes that determine barrier formation. This study identified an ecotype in the AA-genome wild-rice O. glumaepatula, distributed in the Amazon River basin, forming an ROL barrier before suffering waterlogging stress. We found several lines forming a constitutive ROL barrier in the introgression lines of O. glumaepatula in the genetic background of O. sativa (cv. Taichung 65). Research has progressed toward identifying the gene(s) responsible for forming the constitutive barrier in wild rice.

Free Research Field

植物分子遺伝学

Academic Significance and Societal Importance of the Research Achievements

酸素漏出バリアは湿生植物がもつ耐湿性の重要形質である。本研究で、過湿ストレスを受ける前から恒常的に酸素漏出バリアを形成する野生イネのエコタイプを特定できた。さらに、この野生イネの染色体断片を栽培イネに導入すると、野生イネ同様に、恒常的にバリアを形成させることができた。既存のイネをモデルとした形成機構の研究では到達できない、バリア形成を決定づける、上流に位置する鍵遺伝子の特定に向けて、研究が進展した。